The D+caf™ Test Strip Caffeine Test
Kit and Caffeine Test Strips is a unique new product that quickly and
easily determines whether your coffee or tea is really decaf or contains
caffeine even in small amounts.
- >98% accurate for detecting “NON-DECAFFEINATED”
beverages - above 20 mg caffeine per 6 oz. serving
D+caf™ is a lateral flow immunoassay, similar to the technology
used in home pregnancy tests
D+caf™ Test Strips can be used on hot or cold beverages
- Produces a result in as little as
- Test beverage before adding milk,
cream, powdered creamers, syrups or other additives
D+caf™ Test Strips please
click here or Call 888-438-1942.
Using the Caffeine
- All “decaf” contains some caffeine - consumers
need to judge for themselves what level of caffeine they are willing
D+caf™ Test Strips allow the caffeine-sensitive consumer to help
make sure they avoid non-decaf beverages
- Caffeine-conscious consumers are recommended
to test all coffee and tea presumed to be “decaf” - BEFORE drinking
We love talking with our customers
about Caffeine Test Kits!
Our normal hours of operation are Monday
through Friday, 9:00 A.M. to 5:00 P.M. Pacific time.
Silver Lake Research Corporation
P.O. Box 686
Monrovia, CA 91017
Toll Free: 1-888-438-1942
Fax: (626) 359-6601
D+caf™ Caffeine Test Strips please
If you are interested in reselling any of our
products please click
Saying about D+CAF Caffeine Test Strips
"When you buy decaf coffee you always
wonder is it really decaf… A lot of people eat dinner at night
and say to the waiter this better be decaf… Here’s a wand out.
It’s called D+CAF Caffeine Test Strips… People need to know
if their coffee is really decaf or they’ll be up all night."
Kathy Lee Gifford and Hoda Kotb,
The Today Show, NBC-TV
"It’s sort of genius! No more inadvertent
caffeine boosts and late-night jitters because of a mislabeled
or otherwise errant cup of java after dinner!" Liz Krieger, Real Simple.com
"Decaf junkies can now test their cups
of java for any unexpected jolt. A new test strip lets coffee
lovers who can’t hack caffeine dip into their cups to make sure
all’s clear – or at least close to caffeine-free." Marina Vataj and David K. Li, New York Post
"This is going to come in handy for those
people who really want to know how much caffeine they’re having."
Live! With Regis & Kelly , WABC-TV
"About 15 percent of coffee drinkers choose
decaf and even though most decaf cups have less caffeine than
regular brew the food and drug administration doesn’t monitor
the levels so you could be getting an unexpected jolt. Now one
savvy research group is aiming to change that." CBS 2 News at 5:00 (NY, NY)
"Now you can be sure you are getting decaf."
Good Day LA KTTV-TV
"The strips are fairly easy to use. Dunk
'em in a teaspoon or less of the suspect beverage and the results
are ready in about a minute." Seattlepi.com, "Health Report"
"If you are pregnant or have other health
concerns involving caffeine such as high blood pressure, you
might want to know if your next cuppa Joe is really decaf."
"While testing your coffee or tea for
caffeine may not be at the top of everyone’s priority list,
it is good to know that products such as D+CAF testing strips
are available for those who have a true need to reduce their
caffeine intake and want to be certain the drinks they purchase
are truly caffeine-free." Skietzman, www.my-best-coffee.com
The Harmful Effects of Caffeine
Should We Use a Caffeine Test Kit?
While attending Fort Valley State University,
I signed up for a class that was held at 8:00am on Monday, Wednesday,
and Friday. The problem with this situation was that I started my party
frenzy on Thursday night and ended it on Monday morning (3:00am). Now
of course I didn’t do this every week but I did it enough to regret
signing up for that class. Getting up on Wednesday morning was not a
problem since I had adequate sleep the night before, however, when Monday
and Friday morning came around it was virtually impossible for me to
get up. When I would finally drag myself out of bed and prepare for
my 8:00 o’clock class (late as usual) I would still have a hard time
staying awake during class.
My roommate suggested that I drink pepsi
or coke before class and the caffeine will keep me awake. Come Friday
morning I did just that. I went to the soda machine conveniently located
across from my class and bought a can of pepsi. While still standing
there I notice the candy machine to my left filled with all kinds of
sweet goodies. “Hmmmm wouldn’t a nice snickers bar go well with this
pepsi?” I asked myself. Without hesitation, I purchase a snickers bar
and headed towards my class.
To my surprise it worked. I was up and alert
during class. However, I was on a caffeine high at 800am and would crash
at 10:00am. I didn’t mind taking an hour nap at 10:00am in exchange
for my power surge during my 8:00 o’clock class.It wasn’t long until I started experiencing some side effects
from the caffeine I was consuming. My first side effect was nervousness.
I couldn’t keep still. When I would sit down I would constantly shake
my leg. I also noticed that it would take more cans of soda to keep
me awake. The second side effect was headaches followed by diarrhea.
In order to get rid of my headache I would drink a cup of coffee. Finally
I was suffering from insomnia. I was a caffeine addict. This is one
of the reasons why I developed uterine fibroids a year later. Fortunately,
during the “detox” program that I was on to help cure myself of cancer,
I slowly weaned myself off sodas and chocolate. My side effects are
gone and I don’t have to worry about developing uterine fibroids again.
I have many concerns about this caffeine.
The major one, possibly even more important than the caffeine itself,
is the toxic chemical used in the many stages of growing and marketing
coffee. The easily rancified oils and the irritating acids contained
in the beans offer further hazards.
Caffeine, like sugar, over stimulates the
adrenals and then weakens them with persistent or chronic use. First,
sugar stimulates and weakens the adrenals, which creates fatigue.
Then we use caffeine to keep us aware and
awake, further depleting our adrenals, to which many respond by drinking
more caffeine with sugar. In addition, people who overuse caffeine tend
to need more tranquilizers and sleeping pills to help them relax or
Caffeine can be a lifetime drug for many.
We begin with hot chocolate or chocolate bars, which contain some caffeine,
move into colas or other soft drinks with caffeine, and then add coffee
Many adults use caffeine daily, but this
is slowly changing with education and experience revealing the long-range
problems resulting from caffeine abuse.
People trying to cut down by drinking decaf
could even be exposed to dangerous chemicals unless they are drinking
coffee prepared by the water process or Swiss process, which uses steam
distillation to remove the caffeine. Otherwise, agents such as TCE (trichlorethylene)
or methylene chloride used in the chemical processing may be contained
as residues in the decaf coffee. Cola naturally contains caffeine, yet
many soft drinks have even more added. The caffeine creates an addiction
to the drink. Xanthines, theophylline, is found in black teas; it is
also commonly used in medicine to aid in breathing. Theobromine, the
third xanthine derivative, is found in cocoa.
Signs and Symptoms of Caffeine Intoxication
nervousnessheadacheincreased heart rate
anxietyupset stomachirregular heartbeat
irritabilityGI irritationelevated blood pressure
Caffeine Withdrawal Symptoms
fatiguedizzinessringing in the ears
depressiondrowsinessfeeling hot and cold
apathy inability to concentrate
The most common withdrawal symptom is a
throbbing and/or pressure headache, usually located at the temples but
occasionally at the back of the head or around the eyes. A vague muscular
headache often follows. Of course, caffeine cures the symptom; but this
is not the answer.
It’s ok to have a piece of chocolate every
now and then but please be sure not to use caffeine on a daily basis,
especially if you are a woman.
Caffeine Test kits could
help consumers avoid caffeine
testing kit that uses antibodies from llama blood could help consumers
and companies identify the presence of caffeine in beverages.
Consumers are increasingly trying to
avoid caffeine due to unwanted health effects, including insomnia
and irritability. The test could be another tool that eventually
leads to a reduction in demand for products containing caffeine.
Chemists at Washington University School
of Medicine in St. Louis are developing a quick, "dipstick" test
that they say could represent the first home testing kit to detect
the common stimulant.
Products do not always indicate whether
they contain caffeine, and the caffeine content of similar food
products can vary widely depending on the manufacturer.
Even drinks that are labeled "decaf"
can contain detectable amounts of caffeine, experts say.
"We envisioned that a simple method to measure
caffeine, even in hot beverages, such as coffee, would be of value
to individuals and institutions wanting to verify the absence of
caffeine," said study leader Jack Ladenson,
a chemist at the university. "This will greatly assist individuals
who wish to avoid caffeine."
Ladenson said he is developing test
strips treated with a specific antibody that react by changing colour
in the presence of caffeine.
The new test will be designed to be
qualitative only, he said. It allows a person to quickly determine
whether caffeine is present, but does not indicate the exact amount
or concentration of caffeine.
In preliminary tests using coffee and
cola, an experimental version of the caffeine test kit effectively
distinguished caffeinated versions of these products from their
decaf counterparts, Ladenson said.
Several studies have linked an increase
in caffeine consumption with a higher risk of miscarriage among
pregnant women. The US Food and Drug Administration has specifically
advised pregnant women to avoid or limit their intake of caffeine.
Current tests to detect caffeine use
sophisticated laboratory methods, including spectroscopy and chromatography.
While caffeine-specific antibodies are commercially available, these
antibodies are destroyed at high temperatures, like those of hot
beverages, and consequently are not practical for use in home tests,
the scientists say.
To develop the new immunoassay test,
Ladenson and his associates obtained an antibody derived from the
blood of llamas. The antibody is resistant to high temperatures
due to its unusually stable structure.
They obtained the antibodies by repeatedly
injecting the animals with caffeine to illicit an immune response
to the drug. The researchers then cloned
the caffeine-specific antibody and combined
it with other chemicals to facilitate caffeine detection.
In early laboratory studies, the antibody
mixture was used to measure the presence of caffeine in both caffeinated
and decaf versions of coffee and cola.
In general, the researchers found that
caffeine test kit results were comparable to those of conventional
chromatography tests and that the caffeine content of these beverages
was accurately labeled.
The antibodies worked equally well in
both hot and cold beverages and did not appear to show any false
readings caused by structures that are similar to caffeine, Ladenson
says. He has filed patents related to the research.
The study will appear in the 1 June
edition of the American Chemical Societies Analytical Chemistry.
Is It Really All That Bad?
Caffeine (kaf-en') - an alkaloid drug,
obtained from coffee and tea, that has a stimulant action, particularly
on the central nervous system. It is used to promote wakefulness
and increase mental activity.
Sources and Related
Caffeine is one in a group of compounds
known as methylxanthines. The other two compounds are theobromine
and theophylline and are found in cocoa and tea, respectively. Caffeine
is found in coffee, tea, cocoa beans, and kola nuts. The following
table lists average amounts of caffeine in the most common sources:
Coffee (10 oz.) brewed, drip
Coffee (10 oz.) instant
Coffee (10 oz.) decaf
Tea (10 oz, brewed)
Iced tea (12 oz)
Hot cocoa (8 oz)
Soft drink (12 oz) Coca Cola
Anacin (2 tablets)
Exedrin (1 tablet)
No Doz (1 tablet)
The Physiology of
Caffeine is absorbed rapidly and is
distributed throughout the body water within about an hour. It has
a metabolic half life of about three hours and is excreted as a
methylxanthine derivitive (in both urine and to a lesser extent,
feces). Caffeine is a cardiac muscle stimulant, smooth muscle relaxant,
and a central nervous system (CNS) stimulant. One mechanism behind
the latter is an increase in the excitiability of neurons in the
CNS. Caffeine also acts as a diuretic, stimulates gastric acid secretions,
and increases plasma glucose and free fatty acid concentrations.
These physiological effects of caffeine are related to both the
drawbacks and benefits of this drug.
The Drawbacks of
Dehydration is a major drawback
of caffeine consumption, and results from the drugs ability
to increase urine production. If you are not careful about replacing
fluids with your morning cup of joe, you could end up dehydrated
pretty quickly - especially in our already-dry environment.
Now would be a good time to reread the Nutrition Paige regarding
In addition to dehydration, caffeine
causes some people to get jittery stomachs or "coffee stomach"
- which can be quite uncomfortable and mask any potential benefits.
Some of the biggest drawbacks of caffeine
have nothing to do with caffeine itself. For example, some people
don't like black coffee and end up putting cream, sugar, flavoring,
etc. into their coffee. A cup of black coffee every day only adds
about 10 calories but a cup of joe with all the bells and whistles
or a specialty drink like a mochas or lattes can run in the hundreds
of calories - not to mention add extra sugar and saturated fat to
For those watching their weight, these
coffee habits can be detrimental. Remember, the powdered non dairy
creamers have coconut oils, which also have saturated fat. Skim
milk and an occasional Equal in place of sugar is a good compromise
if plain black coffee doesn't float your boat.
For some, a cup of coffee in the morning
replaces a healthy breakfast as it tends to curb the appetite. If
this is the case for you, you may want to put off the caffeine for
later and see how much energy a good breakfast gives you!
An obvious drawback to any drug is the
withdrawal symptoms that accompany its abuse. For caffeine, this
includes primarily headache, and nausea and vomiting are more severe
side effects of withdrawal.
The Benefits of Caffeine
An increased alertness and wakefulness
is probably the primary reason people consume caffeine of any
form. BUT...did you know that caffeine's perk-me-up quality
is only effective when you are truly fatigued? In other words,
if you get plenty of sleep, coffee is probably not doing a whole
lot of good - and the perceived alertness is just that... perceived!
(but, there is certainly nothing wrong with the placebo effect
- as long as you can minimize the drawbacks above).
Athletes commonly consume caffeine
for its proven performance enhancing effect. At first it was
thought that because caffeine stimulates the release of fats
into the blood, the working muscles would burn more of these
fats, essentially sparing limited muscle glycogen. This has
never been proven, however. While caffeine does release fats
into the blood, this is probably not significant for competitive
athletes who are already "wired" on adrenaline. A more likely
explanation is the ability of caffeine to make exercise seem
easier due to its stimulant effect on the brain, which may reduce
the fatigue associated with long bouts of exercise.
Who Should Avoid Caffeine?
Caffeine should be avoided by those
with any of the following clinical conditions:
irritable bowel syndrome
This is not meant to be a comprehensive
list by any means, but these are some of the major conditions where
caffeine should be avoided. Also, patients should be made aware
of any caffeine found in medications in case they are sensitive
to certain levels of caffeine.
The Caffeine Bottom
of the studies linking caffeine (coffee consumption) with health
problems are inconsistent. Increased caffeine consumption has been
associated with elevated cholesterol, fibrocystic breast disease,
rapid heart beat, and some cancers. Studies showing either a relationship
or no relationship seems to be about equal in number.
In 1986 the American Journal of Epidemiology
reported a 25 year follow up study on 3000 people which showed that
men who drank more than 5 cups of coffee a day had an increased
mortality - but this was not the case for women.
The jury is obviously still out on the
hazards of caffeine, but most of the studies showing some link between
caffeine and a health risk report levels of more that 4 cups of
coffee a day. In my opinion, caffeine is like anything else - used
in moderation (200 mg a day or so) it is probably harmless.
For those of us who choose to consume that tasty beverage called
joe, it's important to recognize some of the drawbacks and make
sure we minimize them.
If you can't function on less than 4
cups a day - I would definitely consider cutting back slowly down
to a more moderate level.
If you really enjoy your one cup a day
- relax and enjoy!
Caffeine is a natural ingredient of coffee
and tea, and is added to other beverages, such as soft drinks
Caffeine is a member of the alkaloid family;
coffee contains other alkaloids, including xanthine and theophylline
Caffeine is the world’s most consumed drug
It is estimated that more than 100 million
Americans drink a total of 350 million cups of coffee a day
Different coffees contain varying levels of
caffeine depending on their botanical variety. Robusta coffee is
grown at a lower elevation, and has more caffeine than arabica coffee
which is grown at higher elevations
Coffee beans generally contain more caffeine
than tea leaves
Herbal teas are non-caffeinated because they
are made from plants that do not contain caffeine
Caffeine concentrations in coffee and tea
depend on the caffeine content of beans/leaves, brewing time, amount
of beans/leaves used per serving
Non-decaf drip coffee averages 75-150 mg caffeine
per 6 oz serving (American Beverage Association website)
Some coffee can have as much as 500 mg caffeine
Decaf coffee always contains some caffeine
– the average is 6-10 mg per 6 oz serving
Strong tea can have >80 mg caffeine per 8
oz cup; weaker tea has less caffeine than stronger tea; average
for brewed tea is 20-90 mg / 8 oz
Caffeine Health effects
Effects of caffeine include agitation, insomnia,
increased heart rate, anxiety, and other unwanted symptoms
Consumers advised to be careful about caffeine
include those with sleep problems, pregnant women, diabetics, persons
taking certain medications, and others under medical advice to avoid
or limit caffeine
Many people avoid drinking caffeinated beverages
to help prevent insomnia, especially after 3 pm
Some people are sensitive to relatively small
doses of caffeine – less than 15 mg
People drink decaffeinated beverages to avoid
the unwanted symptoms of caffeine
To avoid symptoms, people need to limit the
total amount (total mg) of caffeine that they consume
is the go-to source for anyone concerned with maintaining
a healthy home environment.
Our parent company,
Silver Lake Research Corporation (SLRC), is the California
biotech company that revolutionized the environmental testing
industry by developing simple test kits that bring laboratory
capabilities within everyone’s reach.
Accurate testing, once available only
through long and expensive analytical laboratory procedures,
is now possible with do-it-yourself products such as the Watersafe®
test kits. SLRC and its proprietary technology have made home
testing quick, convenient and affordable. Now,
DiscoverTesting.com brings the accuracy of a laboratory
into the hands of every health conscious consumer.
offers a wide selection of home testing kits and solutions to
contamination problems, products from
Silver Lake Research and other top national brands such
as Brita®, Blueair®, Kidde® and more.
each item is handpicked by a team of scientists and industry
experts, bringing you the most innovative, best quality products
to make your home a safe home.
With experience you can count on and products
you can depend on,
is your one-stop shop for home safety products and solutions.
In a 2002 story on
innovative biosensors, we wrote that “researchers
have long hoped for ways to make cheap and long-lasting
artificial antibodies.” One of the companies that, in
the intervening six years, developed just such a technology
was Silver Lake Research, which claims that it can produce
antibodies geared to any particular molecular target.
Silver Lake has introduced
antibody tests that municipalities can use to assess
water supplies and that commercial farms can use to
look for pathogens in cattle, but the company’s first
consumer product is a test for caffeine in supposedly
decaf coffee and tea. Dip one of its tiny test strips
into a fluid sample, and stripes on the strip will change
color if the sample contains more caffeine than advertised.
you're concerned about caffeine in your diet, help
may be on the way in the form of a dipstick-style
caffeine test strip. Chemists at Washington University
School of Medicine in St. Louis say that the product
would be the first of its kind, according to a story
FoodNavigator. The test strips would be treated
with an antibody, cloned from one found in llama
blood, that changes color when it comes in contact
with caffeine. The strips wouldn't tell how much
caffeine a drink had, only whether or not it was
present. The llama blood antibody was apparently
chosen because it worked well at high temperatures.
Posted November 21, 2008 at 05:05:44
AM by Carrington Fox
I was home sick recently,
and while I lay in bed too weak to change the
channel, I endured endless hours of daytime
TV. I think it was during an enthusiastic exchange
between Kathy Lee and Hoda that I learned about
these handy little strips for testing the
caffeine levels of your coffee.
According to the marketing
propaganda, as many as 30 percent of decaf coffee
orders in restaurants aren't exactly
decaf. I can believe that. After all, it can
be hard to remember whether the orange pot is
the one with or without the
True confessions time:
How many of you have tried to pass off caffeinated
coffee as decaf to your guests—either at home
or in a restaurant?